Figure 3. CAP stimulation evokes aggressive approach in males and females.

(A) CAP activation in GH males and females (i), but not MAP activation in males (ii), promotes approach. Courtship, measured by bouts of wing extension, is not affected by CAP stimulation (iii). Light and dark color lines: individual data and the mean, respectively.

(B) Analysis of approach preference in CAP-stimulated testers towards: (i) flies vs. an inanimate object (magnet); (ii) same- vs. opposite-sex fly targets; and (iii) two female fly targets. The preference index (PI): (i) PI = (n_{toward the fly target} − n_{toward the magnet}) / n_total; (ii), PI = (n_{toward the male target} − n_{toward the female target}) / n_total; (iii), PI = (n_{toward the left female target} − n_{toward the right female target}) / n_total. See also Methods. Dark lines: mean±SEM. Light circles: individual data.

(C) Silencing of CAP neurons using Kir2.1 reduces naturally-occurring aggression in SH males (i) and females (ii). Dark lines: mean±SEM. Light circles: individual data.