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. Author manuscript; available in PMC: 2022 Jan 22.
Published in final edited form as: Biochem Biophys Res Commun. 2020 Dec 26;537:1–6. doi: 10.1016/j.bbrc.2020.12.076

Figure 2. BBK13 to BBK13 interactions are detected by far-western blot.

Figure 2.

(A) Schematic representation of the far-western blot method used to identify recombinant BBK13 interactions. (B) rBBK13-HIS was resolved by SDS-PAGE and transferred to a nitrocellulose membrane. The membranes were probed with either rGST-BBK13 or rGST (negative control). Protein-protein interactions were detected by immunoblot with antibodies against GST (αGST). (C) The nitrocellulose membranes were stripped and reprobed with antibodies against BBK13 (αBBK13). Molecular mass standards are shown in kilodaltons (kDa).