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. Author manuscript; available in PMC: 2021 Jul 18.
Published in final edited form as: Nat Metab. 2021 Jan 18;3(1):33–42. doi: 10.1038/s42255-020-00334-y

Fig. 3. Doxycycline significantly increases the lifespan and fitness of a mouse model of complex I deficiency.

Fig. 3.

a. Body weight of Ndufs4−/− KO and WT mice over time (n=10 WT and KO Chow, n=8 WT and KO Dox 5000 ppm, n=10 KO Dox 8000 ppm, and n=7 WT Dox 8000 ppm mice). b. Survival of Ndufs4−/− mice is significantly increased with dietary doxycycline supplementation at 5000 ppm and 8000 ppm (n=19 Chow, n=21 Dox 5000 ppm, and n=21 Dox 8000 ppm mice) (p<0.0001, log rank test for both treatments compared to chow control). c–e. Rotarod performance of KO and WT mice at 30, 40, and 50 days. Untreated KO mice show a progressive decline in performance which is partially prevented with doxycycline treatment. Maximum time of 300 seconds with 3 attempts per mouse per time point. The maximum time is represented (at least n=5 mice were tested for each treatment at each time point). Data are presented as mean values +/− s.e.m. error bars, Student’s t-test with a two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, with Q = 5%, * q<0.05.