Table 4. Dramatic caspase 3 activation in neurons of EtOH-exposed fetal brains.
In the brains of EtOH-exposed fetuses, there appeared to be fewer cells of all types (at 400x magnification) than in unexposed controls. Co-staining for NeuN and cleaved caspase-3 showed that cell injury was especially common in neurons.
| Controls (n=8) | EtOH Exposure (n=8) | |
|---|---|---|
| Total Cells (DAPI) (%) | 100 | 67 ± 3.2 |
| NeuN (%±SD) | 83 ± 3.7 | 36 ± 2.9 |
| Cleaved Caspase-3 (%±SD) | 19 ± 1.8 | 28 ± 3.1 |
| NeuN + Cleaved Caspase-3 (%±SD) | 9 ± 0.8 | 21 ± 4.4 |