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. Author manuscript; available in PMC: 2021 Jun 23.
Published in final edited form as: Nature. 2020 Dec 23;589(7843):591–596. doi: 10.1038/s41586-020-03057-y

Figure 3. RAMP1 signals directly in HSCs.

Figure 3.

a. Quantification of Ramp1 mRNA levels in sorted cell populations from the BM by qRT-PCR. n=3-4 biological samples. b, Schematic illustration of the macrophage depletion experiment. c, Macrophage numbers (Gr-1−F4/80+CD115intSSCint/lo) per femur from PBS or clodronate-liposome-treated mice implanted with osmotic pumps containing saline or CGRP. n=7 mice per group. d, CXCL12 levels in BMEF measured by ELISA. n=7 biological samples. e, f, HSCs (Lin−Sca-1+cKit+CD150+CD48−) and LSKs (Lin−Sca-1+cKit+) per mL of peripheral blood. n=7 mice per group. g, Experimental design of the transwell assay. h, Colony-forming units per well of migrated stem progenitor cells from Ramp1+/+ (WT) or Ramp1−/− (KO) mice. n=5, 4 mice, respectively. i, Schematic illustration of the mixed chimerism experiment. j, Chimerism of mobilized HSC in blood normalized to the chimerism of BM HSCs. n=4,4,3,3 mice, respectively. Error bars represent s.e.m. Two-tailed unpaired Student’s t test (c-e, h) or one-way ANOVA (j).