Extended Data Fig. 4. G-CSF-induced HSPC mobilization is impaired in mice lacking nociceptor neurons.

a, White blood cell counts and absolute numbers of LSK (Lin⁻ Sca-1⁺ c-Kit⁺) cells per mL of peripheral blood following G-CSF mobilization in vehicle-treated control and RTX-treated mice. n=11, 13 mice, respectively. b, Spleen weight and the absolute numbers of LSK cells in the spleen following G-CSF mobilization in vehicle-treated control and RTX-treated mice n=11 mice per group. c, Bone marrow cellularity and the absolute numbers of LSK cells in the bone marrow following G-CSF-induced mobilization in vehicle-treated control and RTX-treated mice. n=11, 13 mice, respectively. d, Cell cycle analysis of BM HSCs (Lin⁻ Sca-1⁺ c-Kit⁺ CD150⁺ CD48⁻) from control or RTX-treated animals determined by FACS using anti-Ki67 and Hoechst 33342 staining. n=6, 4 mice, respectively. e, White blood cell counts and absolute numbers of LSK cells per mL of peripheral blood following G-CSF-induced mobilization in Na_v1.8-Cre⁻; iDTA⁺ and Na_v1.8-Cre⁺; iDTA⁺. n=4, 5 mice, respectively. f, Bone marrow cellularity and the absolute numbers of LSK cells in the bone marrow following G-CSF-induced mobilization in Na_v1.8-Cre⁻; iDTA⁺ and Na_v1.8-Cre⁺; iDTA⁺. n=4, 5 mice, respectively. g, Peripheral blood B-cell (B220⁺CD45.2⁺), blood T-cell (CD3e⁺CD45.2⁺), and myeloid-cell (Mac-1⁺CD45.2⁺) donor chimerism in CD45.1-recipient mice transplanted with mobilized blood (CD45.2) derived from saline or RTX-treated mice mixed with CD45.1 competitor BM cells at the indicated time points post-transplantation. n=9,8 mice, respectively. h, Total bone marrow chimerism (CD45.2⁺) and bone marrow HSC chimerism (Lin⁻ Sca-1⁺ c-Kit⁺ CD150⁺ CD48⁻ CD45.2⁺) 20 weeks after transplantation. n=9, 8 mice, respectively. Error bars represent s.e.m. Two-tailed unpaired Student’s t test. For box plots, the box spans from the 25th to 75th percentiles and the centerline was plotted at the median. Whiskers represent minimum to maximum range.