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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Dev Biol. 2020 Dec 4;471:10–17. doi: 10.1016/j.ydbio.2020.11.011

Figure 1. Cep70 is a deuterosome associated protein.

Figure 1.

A–B, Z-projections of a non-MCC (A) and an MCC (B) labeled with the centriole marker Centrin4-RFP (red) and GFP-Cep70 (green), inlays are side projections. Scale bar if 5μm. C–D, Mature MCCs in embryos injected with RFP-Cep70 and GFP-CCDC78 (C) and GFP-Deup1 (D). C’–D’, side projections of C–D with arrows identifying colocalization below the apical surface. Scale bar is 5μm. E, Co-IP using FLAG beads to pull down FLAG-CCDC78 followed by an anti-GFP western blot to identify GFP-Cep70. F, Co-IP using FLAG beads to pull down FLAG-Cep70 followed by anti-GFP western blot to identify GFP-CCDC78. G, Co-IP using FLAG beads to pull down either FLAG-CCDC78 (lanes 1–3) or FLAG-Cep70 (lanes 4–6) followed by anti-GFP western blot to identify GFP-Deup1.