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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Exp Eye Res. 2020 Nov 29;202:108370. doi: 10.1016/j.exer.2020.108370

Fig. 1. Efficient induction of Cre activity in the RPE of both sexes over a range of ages.

Fig. 1.

(A) Schematic of the DNA construct used to generate the Rosa26BEST1-CreERT2 allele by ϕ C31-mediated integration. Rosa26BEST1-CreERT2 mice were subsequently crossed to Ai14 mice with a Cre-conditional tdTomato reporter cassette at Rosa26 to generate Rosa26 BEST1-CreERT2/ LSL-tdTomato mice. Cre-mediated recombination excises the stop region, enabling tdTomato expression. (B) tdTomato fluorescence of RPE/choroid/sclera flatmounts from Rosa26 BEST1-CreERT2/ LSL-tdTomato mice of each sex injected with 4OHT for four consecutive days beginning at P14 (30 mg/kg) or at 7 weeks of age (60 mg/kg). White boxes indicate areas of higher magnification shown in the insets. (C) The efficiency of induced Cre-mediated recombination was quantified as the mean ± S.E. percentage of the total RPE flatmount area that was tdTomato positive. n = 3 eyes from different animals for each category. The efficiency was not statistically different by one-way ANOVA. (D) Retinal cryosection from a 4OHT treated (four 30 mg/kg injections beginning at P14) male Rosa26 BEST1-CreERT2/ LSL-tdTomato mouse. Scale bars: (B) 1000 μm, (C) 170 μm