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. Author manuscript; available in PMC: 2021 Feb 3.
Published in final edited form as: Bioconjug Chem. 2020 Dec 9;32(1):82–87. doi: 10.1021/acs.bioconjchem.0c00557

Figure 1.

Figure 1.

Traditional production of semisynthetic RNase S. Step 1: Proteolytic digestion of RNase A to cleave S-peptide (residues 1–20) and yield catalytically inactive S-protein (residues 21–124). Step 2: Reversible association of S-protein and a synthetic S-peptide variant to produce catalytically active, semisynthetic RNase S. Images were produced with PyMOL software and PDB entries 1jvt21 and 1rnu.22