Figure 3.

(A) Scheme for the semisynthesis of human RNase–S. Image was produced with PyMOL software and PDB entry 1z7x.³⁹ (B) Graph showing the ribonucleolytic activity of RNase–S (5 nM) with a fluorogenic substrate, 6-FAM–dArUdAdA–6-TAMRA (200 nM), in 0.10 M DEPC-treated⁴¹ OVS-free⁴² MES–NaOH buffer, pH 6.0, containing NaCl (0.10 M); λ_ex 493 nm, λ_em 515 nm. The assay was performed in triplicate.