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(A) A5C S-peptide bearing N-terminal pendants. (B) Coomassie staining and fluorescence imaging of alkynyl RNase–S following CuAAC with 5-TAMRA–azide and SDS–PAGE. (C) Antibiotin immunoblot analysis of biotinylated RNase–S. (D) Coomassie staining and fluorescence imaging of fluorescein-tagged RNase–S after SDS–PAGE. WT, wild-type RNase 1.
