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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Dev Biol. 2020 Dec 5;471:1–9. doi: 10.1016/j.ydbio.2020.12.001

Fig. 6. Hoxb5b function in the endoderm is not necessary for midline coalescence of the foregut endoderm.

Fig. 6.

Cell transplantation approach: Tg(sox17:EGFP) embryos were used to follow the fate of endodermal cells in chimeric embryos. (A) Confocal image of a Sox32 morphant embryo; these lack endoderm and were used as hosts. (B) Tg(sox17:EGFP) donor embryos were injected with sox32 mRNA, or (C) together with Hoxb5b morpholino. Donor cells were transplanted into endoderm-deficient Sox32 morphant hosts. Whole mount immunolabeling for GFP (green), Myosin to label somites (magenta) and nuclear marker (blue). Both wild-type (WT) (B) and Hoxb5b morphant (C) donor cells reconstitute a normal gut in Sox32 morphant hosts (A). Anterior to the left, results are from 2 independent experiments with a minimum of 4 embryos per group.