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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Biomaterials. 2020 Dec 1;268:120565. doi: 10.1016/j.biomaterials.2020.120565

Figure 1.

Figure 1.

Immune responses during fibrotic capsule formation around a subcutaneously implanted Teflon mandrel. To make fibrotic conduits, Teflon mandrels were implanted into the subcutaneous pouches in the abdominal wall of rats. The cross sections of fibrotic tissues at days 3, 7 and 28 were analyzed by immunostaining and fluorescence microscopy, (a) Immunostaining for fibroblast markers FSP1 (green) and α-SMA (red). Nuclei were stained by DAPI (blue), (b) Immunostaining for CD68 (Pan-macrophage), CCR7 (M1 macrophage r), CD163 (M2 macrophage), CD3 (T lymphocyte), CD4 (Helper T-cell subset), FoxP3 (Regulatory T-cell) and CD19 (B lymphocyte), (c) Percentage of CD68, CCR7 and CD163 positive cells. (d) Percentage of CD3, CD4 and FoxP3 positive cells, (e) Percentage of CD19 positive cells. Scale bars: 50 μm. Lumen is marked with *. (n=6)