Table 2.

Commonly used techniques to separate exosomes from milk, cells, and biological fluids.

Technique	Principle	Purity	Yield	Advantage	Disadvantage
ExoQuick precipitation	Polymer based precipitation	Low	1 mL	Easy Method	High cost
Ultracentrifugation	Differential centrifugation	High to low	100–500 mL	High yield	Time consuming
Density gradient ultracentrifugation	Differential centrifugation	High	10 mL	High purity	Time consuming
Isoelectric precipitation	Precipitation at isoelectric point	Low	10–20 mL	Low cost	Low purity
Size exclusion chromatography	Porous columns filled with polymers	High	10–100 mL	High yield of exosomal proteins	Complex Method