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. 2021 Jan 29;13:871–887. doi: 10.2147/CMAR.S250778

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© 2021 Wang et al.

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Autophagy was activated by GAS5 in AMC-HN-8 cells. The protein expressions of LC3I, LC3II, Beclin1 and p62 in AMC-HN-8 cells were determined (A) and quantified (B) by Western blot. The protein expressions of LC3I, LC3II, Beclin1 and p62 in Tu 177 cells were determined (C) and quantified (D) by Western blot. (E) The mRNA levels of LC3I, LC3II, Beclin1 and p62 in AMC-HN-8 cells were determined by qRT-PCR. (F) The mRNA levels of LC3I, LC3II, Beclin1 and p62 in Tu 177 cells were determined by qRT-PCR. **P<0.01 vs Blank group, ^##P<0.01 vs NC .

Abbreviations: siNC, silenced negative control; NC, negative control; GAS5, long noncoding RNAs growth arrest-specific 5; siGAS5, silenced Long noncoding RNAs growth arrest-specific 5; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.