Figure 9.
Overexpression of ULK2 rescued the inhibitory effects of miR-26a-5p mimic on AMC-HN-8 cells proliferation. Transfection efficiency of ULK2 in AMC-HN-8 cells was confirmed (A) and quantified (B) by Western blot. Transfection efficiency of ULK2 in Tu177 cells was confirmed (C) and quantified (D) by Western blot. (E) AMC-HN-8 cells viability was tested by CCK-8 assay. (F) Tu177 cells viability was tested by CCK-8 assay. **P<0.01 vs MC+NC or IC+siNC, ##P<0.01 vs M+NC or I+siNC.
Abbreviations: M, mimic; MC, mimic control; I, inhibitor; IC, inhibitor control; siNC, silenced negative control; NC, negative control; OD, optical density; GAS5, long noncoding RNAs growth arrest-specific 5; siGAS5, silenced Long noncoding RNAs growth arrest-specific 5.