Fig. 7.

Cyclic peptides targeting NET-resident H2A disrupt monocyte adhesion that is promoted by NETs. In vitro static adhesion assay to monitor monocyte adhesion to NETs. Human classical monocytes were added to immobilized neutrophils (ctrl) or to NETting neutrophils (induced by A23187) and monocyte adhesion was quantified in a fluorescence plate reader as mean fluorescence intensity (MFI). To study monocyte adhesion to NETs and to investigate the ability of cyclic-6, −7, −8, −9 and −17 (CHIP) to block this process, NETs were preincubated with these peptides at the concentration of 200 µg/ml. Data are mean +/- SEM. One-way Anova. **p < 0.01, *<0.05 vs. NETs. n = 5–14 per bar.