Fig. 1.

Acquisition of a confocal image suitable for quantification Panel A is a representative confocal image of a section taken through the adult mouse lens epithelium stained for E cadherin (Alexa Fluor 488, green) and DNA (DRAQ5, blue) imaged with laser, master gain, and offset settings appropriate for quantitation as shown in the “range indicator” view of this image shown in Panel B which shows that areas lacking tissue have a very little signal (blue background), while few to no red pixels (signifying saturation of the detector) are seen. Panel C shows a negative control section through the lens epithelium that was created by omitting the primary antibody in the experiment, and Panel D shows the range indicator view of the negative control image Panel E is also a confocal image of a section taken through the adult mouse lens epithelium stained for E cadherin (Alexa Fluor 488, green) and DNA (DRAQ5, blue), however, the settings used have oversaturated the green channel as shown by the large number of “red” pixels seen in the range indicator view shown in Panel F. C- Lens Capsule, LC- Lens cells, Scale bar- 35 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)