Fig. 2.

Quantitation of signal intensity in a region of interest using the Mean fluorescence intensity (MFI) method (Approach 1 (3.2.1)) A. Original confocal image of a section cut through an adult lens epithelium ((E cadherin (Alexa Fluor 488- green), αSMA (Cy3-Red) & DNA (DRAQ5-blue)). B. View showing the channel containing the E cadherin to be quantified. C. Selection of the region of interest by tracing the tissue using a high-resolution drawing tablet, and output from MFI measurement. D. Determination of the MFI of the background from a rectangular area of the image that lacks tissue and the output from that measurement. C- Lens Capsule, LC- Lens cells, Scale bar- 35 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)