Table 1.
Examples of ImageJ quantitation method validation with other analytical approaches post cataract surgery (PCS).
| Gene ID and description | Transcriptome analysis (RNAseq) | Fiji quantitation | Additional analytical tools | Remarks and references |
|---|---|---|---|---|
| Acta2 actin, alpha 2, smooth muscle, aorta (αSMA) | 0 h vs 48 h FC- (4.4) FDR- 3.39E-08 |
0 h vs 3 d FC- (8.87) ***P < 0.001 0 h vs 5 d FC- (6.35) *P = 0.013 |
Fluorescent activated cell sorting (FACS) 0 h vs 5 d FC- (2.92) ***P < 0.001 |
Consistent and validated [1] |
| Cdh1 cadherin 1 (E− cadherin) | 0 h vs 48 h FC- (−2.12) FDR- 7.0E-05 |
0 h vs 48 h FC- (−1.68) *P = 0.019 |
Qualitative immunofluorescent image observation 0 h vs 5 d Downregulation
|
Consistent and validated [1] |
| Col1a1 Collagen, type I, alpha 1 |
0 h vs 48 h FC- (83) FDR- 2.1E-42 |
0 h vs 48 h FC- (7.35) ***P < 0.001 |
Qualitative immunofluorescent image observation 0 h vs 48 h Robust expression/upregulation
|
Consistent and validated [1] |
| Tnc tenascin C | 0 h vs 48 h FC- (116) FDR- 7.7E-44 |
0 h vs 48 h FC- 12.78 **P = 0.005 |
qRT-PCR 0 h vs 24 h ***P < 0.0001 |
Consistent and validated [1,18] |
h-hour, d-day, FC- fold change, FDR-false discovery rate, FACS- Fluorescence-activated cell sorting, P - probability value, qRT-PCR- Real-Time Quantitative Reverse Transcription PCR.
-- Upregulation, -- Downregulation.