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. 2021 Feb 1;2(1):100314. doi: 10.1016/j.xpro.2021.100314

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Transplantation of metanephroses with ureters and bladder (MNBs) containing exogenous renal progenitor cells (RPCs) to host animals

(A–C) Detachment of MNBs.

(A) Both groins are dissected vertically.

(B) Micro-tweezers are inserted under the metanephroses (MNs) to peel off the connective tissue.

(C) The MNs are lifted, and the bladder remains connected to the body (arrow).

(D) The MNB is detached and trimmed. Injected Green fluorescent protein-tagged renal progenitor cells (GFP-RPCs) are confirmed.

(E) The MNB is transplanted into the pocket of the retroperitoneum in the vicinity of the descending aorta in a NOD/Shi-scid, IL-2RγKO Jic (NOG) mouse. Scale bar, 1 mm.