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. 2021 Feb 1;2(1):100314. doi: 10.1016/j.xpro.2021.100314

Figure 7.

Figure 7

Representative images of expected outcomes

(A) Immunostaining of a frozen section of metanephroses with ureters and bladder (MNBs) that contain exogenous mouse renal progenitor cells (RPCs) 2 weeks after transplantation (in vivo). Green fluorescent protein (GFP)-positive neo-glomeruli originating from the transplanted RPCs are confirmed. Scale bar, 50 μm.

(B) Hematoxylin and eosin (HE) staining and immunostaining of a frozen section of MNBs containing exogenous rat RPCs 4 weeks after transplantation (in vivo). Fluorescence microscope analysis revealed that dextran is filtrated by GFP-positive glomerular and tubular structures of neo-nephrons. Scale bar, 500 μm.

(C) According to flow cytometry, 10%–40% of the nephron progenitor cell spheres (NPSs) induced from human induced pluripotent stem cells (hiPSCs) are ITGA8+/PDGFRA− pure nephron progenitor cells (NPCs).