REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Polyclonal rabbit anti-Six2 antibody	Proteintech	Cat# 11562-1-AP; RRID: AB_2189084
Monoclonal rat anti-CK8 (Cytokeratin 8) antibody	DSHB	Cat# TROMA-I; RRID: AB_531826
Polyclonal rabbit anti-WT1 antibody	Santa Cruz Biotechnology	Cat# sc-68880; RRID: AB_2241672
Polyclonal goat anti-Nephrin antibody	Santa Cruz Biotechnology	Cat# sc-19000; RRID: AB_650006
Polyclonal rabbit anti-CDH6 antibody	Sigma-Aldrich	Cat# HPA007456; RRID: AB_1078373
Polyclonal rabbit anti-AQP1 (Aquaporin 1) antibody	Millipore	Cat# AB2219; RRID: AB_1163380
Polyclonal goat anti-Megalin antibody	Santa Cruz Biotechnology	Cat# sc-16478; RRID: AB_2234897
Monoclonal mouse anti-ECAD (E-Cadherin) antibody	BD	Cat# 610181; RRID: AB_397580
Polyclonal rabbit anti-CD31 antibody	Abcam	Cat# ab28364; RRID: AB_726362
Monoclonal rabbit anti-Pax8 antibody	Abcam	Cat# ab189249; RRID: AB_2801268
Polyclonal rabbit anti-GFP (green fluorescent protein) antibody	MBL	Cat# MBL598; RRID: AB_591819
Polyclonal chicken anti-GFP antibody	Abcam	Cat# ab13970; RRID: AB_300798
Monoclonal mouse anti-Nuclei antibody	Millipore	Cat# MAB1281; RRID: AB_94090
Monoclonal mouse anti-Vimentin [V9] antibody	Abcam	Cat# ab8069; RRID: AB_306239
Biotinylated polyclonal goat anti-mouse ITGA8 antibody	R&D	Cat# BAF4076; RRID: AB_2128449
APC-conjugated streptavidin	BioLegend	Cat# 405207
PE-conjugated monoclonal mouse anti-human PDGFRα antibody	BioLegend	Cat# 323506; RRID: AB_2268113
Chemicals, peptides, and recombinant proteins
MEM α	Gibco	Cat# 12561056
Accutase	Innovative Cell Technologies	Cat# AT104-500
Fetal bovine serum	GE Healthcare Life Science	Cat# SH3007.03
iMatrix-511	Nippi	Cat# 892012
Stemfit AK02N	Ajinomoto	Cat# RCAK02N
Y27632	Wako	Cat# 257-00511
Activin A	R&D	Cat# 338-AC
FGF-basic	R&D	Cat# 233-FB
CHIR	Axon	Cat# AXN1386-00
Bmp4	R&D	Cat# 314-BP
Retinoic acid	Sigma-Aldrich	Cat# R-2625-50MG
FGF-9	R&D	Cat# 273-F9-02
Neuron Dissociation Solutions kit	Wako	Cat# 291-78001
DMEM/F12	Gibco	Cat# 11320033
B-27 minus vitamin A	Gibco	Cat# 12587010
GlutaMAX	Gibco	Cat# 35050061
Insulin-transferrin-selenium solution	Gibco	Cat# 41400045
MEM non-essential amino acids solution (100×)	Gibco	Cat# 11140050
2-Mercaptoethanol	Gibco	Cat# 21985023
Penicillin-streptomycin (5,000 U/mL)	Gibco	Cat# 15070063
4-Hydroxytamoxifen	Sigma-Aldrich	Cat# H7904-5MG
Tamoxifen	Sigma-Aldrich	Cat# T5648-1G
Corn oil	Wako	Cat# 032-17016
Isoflurane	Pfizer	N/A
Pentobarbital	Kyoritsu Seiyaku	N/A
Tacrolimus	Astellas Pharma	N/A
Methylprednisolone	Pfizer	N/A
Tetramethylrhodamine-labeled dextran	Invitrogen	Cat# D1817
TWEEN 20	Sigma-Aldrich	Cat# P1379-100ML
Donkey serum	Jackson ImmunoResearch	Cat# 017-000-001
Skim milk	Wako	Cat# 190-12865
Triton X-100	Nacalai Tesque	Cat# 12967-32
HistoVT One	Nacalai Tesque	Cat# 06380-05
Blocking One Histo	Nacalai Tesque	Cat# 06349-64
Signal Enhancer HIKARI for Immunostain Solution A	Nacalai Tesque	Cat# 02373-54
Critical commercial assays
RNeasy Plus Micro kit	QIAGEN	Cat# 74034
PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time)	TAKARA	Cat# RR047A
TaqMan Gene Expression Master Mix	Applied Biosystems	Cat# 4369016
Six2 (Rn01769531_m1) TaqMan Assay	Applied Biosystems	N/A
Nphs1 (Rn00674268_m1) TaqMan Assay	Applied Biosystems	N/A
Nphs2 (Rn00709834_m1) TaqMan Assay	Applied Biosystems	N/A
Lrp2 (Rn00578067_m1) TaqMan Assay	Applied Biosystems	N/A
Aqp1 (Rn00562834_m1) TaqMan Assay	Applied Biosystems	N/A
Slc12a1 (Rn00692576_m1) TaqMan Assay	Applied Biosystems	N/A
Scnn1a (Rn00580652_m1) TaqMan Assay	Applied Biosystems	N/A
Gapdh (Rn01775763_g1) TaqMan Assay	Applied Biosystems	N/A
Experimental models: cell lines
201B7 human iPSC line	RIKEN BRC	Cat# HPS0063
Experimental models: organisms/strains
Mouse: B6;129-Gt(ROSA)26Sor[tm1(DTA) Mrc]/J (Rosa26-DTA176)	Jackson Laboratory	Cat# 010527
Mouse: B6;129-Six2[tm3(EGFP/cre/ERT2) Amc]/J (Six2-GCE)	Jackson Laboratory	Cat# 009600
Mouse: C57BL/6-Tg (CAG-EGFP)	SLC Japan	N/A
Mouse: NOD/Shi-scid, IL-2RγKO Jic (NOG)	CLEA Japan	N/A
Rat: Sprague-Dawley-Tg (CAG-EGFP)	SLC Japan	N/A
Rat: LEW/SsNSlc (Lewis)	SLC Japan	N/A
Software and algorithms
Prism7	GraphPad	https://www.graphpad.com/scientific-software/prism/
Fiji	Schindelin et al., 2012	https://imagej.net/Fiji
Other
Fluorescence stereo microscope	Leica Microsystems	Cat# M205FA
Greenough stereo microscope	Leica Microsystems	Cat# S9D
MACSQuantify	Miltenyi Biotec	Cat# 130-094-556
LSM 880 with Airyscan	ZEISS	Cat# LSM880
All-in-one fluorescence microscope	KEYENCE	Cat# BZ-X800
40-μm cell strainer	Corning	Cat# 352340
12 mm Transwell	Corning	Cat# 3401
Microplate for tissue culture (for adhesion cell) 6-well	IWAKI	Cat# 3810-006
V-bottom 96-well low-cell-binding plates	Sumitomo Bakelite	Cat# MS-9096V
U-bottom 96-well low-cell-binding plates	Thermo Scientific	Cat# 174929
SlowFade Diamond Antifade Mountant	Invitrogen	Cat# S36972
Prolong Gold Antifade Mountant with DAPI	Invitrogen	Cat# P36931
Tissue-Tek OCT compound	Sakura Finetechnical	Cat# 4583
PLATINUM PRO glass slide	Matsunami Glass Ind., Ltd.	Cat# SPRO-04
Thin-walled glass capillary	NARISHIGE	Cat# G-100
Puller	NARISHIGE	Cat# PC-10
Aspirator tube assembly	Drummond Scientific Company	Cat# 2-040-000
Pen-type syringe pump	Takasago Fluidic Systems	Cat# SBP-100G-LL