10× HEPES stock (store at 4°C; lasts about 1 month)
| Reagent | Final (1×) concentration (mM) | 10× concentration (mM) | Amount for 1 L |
|---|---|---|---|
| KCl | 2.5 | 25 | 1.86 g |
| NaH2PO4 | 1.25 | 12.5 | 1.50 g |
| NaHCO3 | 30 | 300 | 25.2 g |
| HEPES | 20 | 200 | 47.6 g |
| ddH2O | – | – | Add to 1 L |
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