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1× NMDG solution (for cutting; store at 4°C; lasts about 1 week). NMDG solution reduces excitotoxicity during the slicing procedure by replacement of sodium ions (Ting et al., 2014)
| Reagent | Stock (mM) | Final concentration (mM) | Amount for 1 L |
|---|---|---|---|
| 10× HEPES stock | 10× | 1× | 100 mL |
| NMDG | – | 92 | 17,960 mg |
| Glucose | – | 25 | 4,505 mg |
| Sodium ascorbate | – | 5 | 990 mg |
| Thiourea | – | 2 | 152.2 mg |
| Sodium pyruvate | – | 3 | 330 mg |
| MgSO4 | 1,000 | 10 | 10 mL |
| CaCl2 | 1,000 | 0.5 | 0.5 mL |
| ddH2O | – | – | ∗Add to 900 mL |
