Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
1× HEPES Holding (for recovery. Store at 4°C; lasts about 1 week) (Ting et al., 2014)
| Reagent | Stock (mM) | Final concentration (mM) | Amount for 1 L |
|---|---|---|---|
| 10× HEPES stock | 10× | – | 100 mL |
| NaCl | – | 92 | 5,372 mg |
| Glucose | – | 25 | 4,505 mg |
| Sodium ascorbate | – | 5 | 990 mg |
| Thiourea | – | 2 | 152.2 mg |
| Sodium pyruvate | – | 3 | 330 mg |
| MgSO4 | 1,000 | 2 | 2 mL |
| CaCl2 | 1,000 | 2 | 2 mL |
| ddH2O | – | – | ∗Add to 900 mL |
