| Reagent | Stock (mM) | Final concentration (mM) | Amount for 500 mL |
|---|---|---|---|
| 10× ACSF stock | 10× | 1× | 50 mL |
| CaCl2 | 1,000 | 2.2 | 1,100 μL |
| MgCl2 | 1,000 | 1.1 | 550 μL |
| ddH2O | – | – | Add to 500 mL |
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