REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
AAV2-hSyn-DIO-hM3Dq-mCherry	Addgene	RRID: AddGene_44361
AAV2/5-GFAP-GCaMP6-LCK	Addgene	RRID: AddGene_52924
AAVDJ-CaMKII-GCaMP6s	Stanford Vector Core	Cat# GVVC-AAV-88
AAV9-Syn-DIO-jGCaMP7f-WPRE	Addgene	RRID: AddGene_104488
Chemicals, peptides, and recombinant proteins
HEPES	Sigma	Cat# H3375
N-Methyl-D-glucamine (NMDG)	Sigma	Cat# M2004
Sodium ascorbate	Sigma	Cat# A7631
Thiourea	Sigma	Cat# T8656
Sodium pyruvate	Sigma	Cat# P5280
N-Acetyl-L-cysteine	Sigma	Cat# A7250
CNO	NIH	Cat# C-929
TTX	Tocris	Cat# 1069
Experimental models: organisms/strains
Mouse: CCK-IRES-Cre	Jackson Laboratory	RRID: IMSR_JAX:012706
Software and algorithms
Micromanager	Micro-manager.org	RRID: SCR_000415
FIJI	Image J	RRID: SCR_002285
Igor Pro	Wavemetrics	RRID: SCR_000325
Other
Small animal stereotaxis apparatus	Kopf	Cat# Model 942
Stereo zoom microscope	Leica	Cat# M80
Rotary drill	Foredom	Cat# MH-170
Capillary glass	WPI	Cat# 1B100-4
Microforge (microelectrode puller)	Narishige	Cat# PC-10
Infusion pump	Kd Scientific	Cat# 78-8130
Gastight syringe 5 μL	Hamilton	Cat# 80016
33 gauge needle (optional)	Hamilton	Cat# 7762-06
Osmometer	Wescor	Cat# Vapro 5520
pH meter with temperature probe	Fisher	Cat# Accumet AE150
Semiautomatic vibratome slicer	Leica	Cat# VT1200S
SliceScope recording microscope	Scientifica	Cat# SliceScope Pro 1000
40× water fluorescence objective	Olympus	Cat# LUMPLFLN40XW
RC-27LD ultra quiet imaging chamber	Warner	Cat# 64-1532
Slice anchor	Warner	Cat# 64-1551
Accuflow flow regulator infusion set	EMED Technologies	Cat# FLOW-100
Optimos sCMOS camera	Q-Imaging	Cat# 01-OPTIMOS-R-M-16-C
Blue LED (470 nm)	CoolLED	Cat# pE-100