Skip to main content
. 2021 Jan 8;10:595533. doi: 10.3389/fonc.2020.595533

Figure 4.

Figure 4

The lncRNA-PAICC promotes proliferation, promotes invasion and regulates YAP1 expression in ICC cells in vitro. (A) Relative expression of lncRNA-PAICC in ICC cell lines by RT-PCR. (B) CCK-8 assays were used to examine cell viability of HuCCT-1 and QBC-939 cells after transfection. (C) Colony formation assays were used to detect the clone ability of HuCCT-1 and QBC-939 cells after transfection. (D) Wound healing assays were shown in HuCCT-1 and QBC-939 cells after transfection. (E) Transwell assays were used to detect cell migration and invasion capacities of HuCCT-1 and QBC-939 cells after transfection. (F) RT-PCR and western blotting were used to detect mRNA and protein expression levels of YAP1 in HUCCT-1 and QBC-939 after knockdown and overexpression of lncRNA-PAICC, respectively. (A–F) The data were Data are mean ± SD. Data between two groups were tested using independent sample t-test. *P < 0.05. All the experiments were repeated three times.