Skip to main content
. 2021 Jan 8;10:595533. doi: 10.3389/fonc.2020.595533

Figure 6.

Figure 6

lncRNA-PAICC exerts its competitive endogenous RNA function by directly sponging microRNAs. (A) The top image shows the predicted binding sites for miR-141-3p and miR-27a-3p in lncRNA-PAICC. The following image shows miR-141-3p and miR-27a-3p binding sites predicted in the transcripts of lncRNA-PAICC. Target mutation underlined. (B) The correlation between lncRNA-PAICC transcript level with miR-141-3p (upper panel) and miR-27a-3p (low panel) transcript level was detected in 76 ICC tissues, respectively. The 2−△△Ct values were subjected to Spearman’s correlation analysis. (C) These two microRNAs relative expression levels in HuCCT-1 and QBC-939 cells after transfection via RT–PCR. Data are mean ± SD. *P < 0.05. The experiment was repeated three times. (D) Luciferase activity in 293T cells co-transfected with miR-141-3p and miR-27a-3p and luciferase reporters that were empty or contained lncRNA-PAICC or the indicated mutant transcript. Data are mean ± SD. *P < 0.05. The experiment was repeated three times. (E) The schematic diagram of the MS2-RIP experiment is shown. After MS2-RIP, RT-PCR was used to detect miRNAs that can endogenously bind to lncRNA-PAICC, and the negative control was miR-150.