REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CTCF	EMD Millipore	Cat# 07-729; RRID: AB_441965
Rabbit IgG, plasma	EMD Millipore	Cat# 401590; RRID: N/A
STARD10	Santa Cruz Biotechnology	Cat# sc-54336; RRID: AB_2197780
FCHSD2	Almeida-Souza et al., 2018	N/A
ARAP1	Abcam	Cat# ab99382; RRID: AB_10675661
α-TUBULIN	Sigma-Aldrich	Cat# T5168; RRID: AB_477579)
Bacteria and virus strain
Stbl3 competent cells	Thermo Fisher Scientific	Cat# C737303
10-beta competent cells	New England Biolabs	Cat# C30191
Biological samples
Human islet samples	Carrat et al., 2017	N/A
Chemicals, peptides, and recombinant proteins
3-Isobutyl-1-methylxanthine (IBMX)	Sigma-Aldrich	Cat# I5879
Foskolin	Sigma-Aldrich	Cat# F3917
Sodium Selenite	Sigma-Aldrich	Cat# S1382
Nicotinamide	Sigma-Aldrich	Cat# 481907
Paraformaldehyde	Agar Scientific	Cat# AGR1026
TRIzol	Invitrogen	Cat# 15596018
NP40	Sigma-Aldrich	Cat# 13021
Proteinase inhibitor cocktail	Sigma-Aldrich	Cat# 04693132001
Albumin from Bovine Serum Fraction V	Roche Diagnostics	Cat# 10775835001
Human Transferrin	Sigma-Aldrich	Cat# T8158
ECM	Sigma-Aldrich	Cat# E1270
RNase A	Thermo Fisher Scientific	Cat# EN0531
Proteinase K	Sigma-Aldrich	Cat# AM2546
Dynabeads-Protein G	Thermo Fisher Scientific	Cat# 10003D
Dynabeads-Protein A	Thermo Fisher Scientific	Cat# 10008D
ATP, [γ-32P]	Perkin Elmer	Cat# NEG002A
Critical commercial assay
Dual-Luciferase Assay Reporter Assay System	Promega	Cat# E1910
Lipofectamine 2000	Thermos Fisher Scientific	Cat# 11668025
Insulin Ultra-sensitive Assay Kit	Cisbio Bioassays	Cat# 62IN2PEH
High-Capacity cDNA Reverse Transcription Kit	Thermos Fisher Scientific	Cat# 4368814
NE-PER™ Nuclear and Cytoplasmic extraction reagents	Thermos Fisher Scientific	Cat# 78833
Taqman™ Fast advanced master mix	Thermos Fisher Scientific	Cat# 4444553
Fast SYBR™ Green Master Mix	Thermos Fisher Scientific	Cat# 4385612
Phusion High fidelity DNA polymerase	Thermos Fisher Scientific	Cat# F530
T4 polynucleotide kinase	New England BioLabs	Cat# M0201S
Experimental model: cell line
HEK293T	ATCC	Cat# CRL_3216; RRID: CVCL_0063
EndoC-βH1	Univercell-Biosolutions	Cat# N/A; RRID: CVCL_L909
Oligonucleotides
VR_KO1: GACCCCTGTGAGCTCCTCGT	This paper	N/A
VR_KO2: AGCGACCACCAGCTAGGTTT	This paper	N/A
CBS1: GCTTGGGTGGGGGTGCAGCC	This paper	N/A
CBS2: GGGCAGTCAAGGGCACAGGA	This paper	N/A
CBS5: TGCAGAAGAATGGTCACTAG	This paper	N/A
CBS7: AGTCTCGGGATCGACACGTG	This paper	N/A
CBS8: CGGCCACAACCACTAGGGGG	This paper	N/A
R2_KO1: GTGTCCTTCACAGTGGCGAT	This paper	N/A
R2_KO2: ACTCCACCTTGGTATTACAC	This paper	N/A
STARD10-1: AGAGGCCGCCAGCTTCTCCA	This paper	N/A
STARD10-2: AGTCTTGGTCATCGGGCACC	This paper	N/A
FCHSD2-1: GCATCATGCAGCCGCCGCCG	This paper	N/A
FCHSD2-2: GCCCTTACCTTCCTCGGCGG	This paper	N/A
ARAP1-1: TGGGGATGCTGCGCTATCGG	This paper	N/A
ARAP1-2: TGCACCTGGAGCAGTACACG	This paper	N/A
HBB_KO1: GCACCATAAGGGACATGATA	This paper	N/A
HBB_KO2: TTTCCTTACTAAACCGACAT	This paper	N/A
Inter_KO1: TTGAGGTTACATCATTCTAC	This paper	N/A
Inter_KO2: AGTACCTAAAGAGACCCACA	This paper	N/A
Scrabmble_1: GAACTCAACCAGAGGGCCAA	This paper	N/A
Scrabmble_2: GGGAGGTGGCTTTAGGTTTT	This paper	N/A
Recombinant DNA
pMD2.G	Didier Trono (https://www.epfl.ch/labs/tronolab/)	Cat# 12259; RRID: Addgene_12259
pSPAX2	Didier Trono (https://www.epfl.ch/labs/tronolab/)	Cat# 12260; RRID: Addgene_12260
BAC DNA	bacpacresources.org	RP11-101P7
pLenti-CRISPR-RIP-Cas9	Paul Gadue	N/A
pGL3-promoter	Promega	E1761
pRL-Renilla	Promega	E2231
pBlueScript II KS+	Stratagene/Agilent	212207
Software
GraphPad Prism 7	GraphPad Software	RRID: SCR_002798
Illustrator	Adobe	RRID:SCR_010279
Photoshop	Adobe	RRID: SCR_014199