Table 2.

Neutralization Activity of gp120 entry-antagonists against a Panel of HIV-1 Env Pseudoviruses

			IC50 μMa
Subtype	NIH #	ENVs	NBD-14136c	NBD-14273
A	11887	Q259ENV.W6	0.46±0.06	0.2±0.01
	11888	QB726.70M.ENV.C4	0.31±0.07	0.11±0.005
	11890	QF495.23M.ENV.A1	0.44±0.05	0.21±0.02
	11891	QF495.23M.ENV.A3	0.26±0.04	0.11±0.01
		BG505-T332N	0.41±0.03	0.118±0.003
		KNH1144	0.62±0.08	0.11±0.02
A/D	11901	QA790.204I.ENV.A4	0.29±0.01	0.12±0.004
	11904	QA790.204I.ENV.E2	0.41±0.03	0.11±0.02
A2/D	11905	QG393.60M.ENV.A1	0.34±0.02	0.13±0.003
	11906	QG393.60M.ENV.B7	0.6±0.005	0.127±0.005
A/G	11591	CRF02_AG Clone 211	0.58±0.05	0.22±0.04
	11594	CRF02_AG clone 250	0.41±0.01	0.13±0.005
	11595	CRF02_AG clone 251	0.36±0.06	0.11±0.01
	11598	CRF02_AG clone 255	0.33±0.01	0.13±0.01
	11599	CRF02_AG clone 257	0.39±0.01	0.13±0.006
	11600	CRF13_cpx clone 258	0.52±0.07	0.175±0.03
	11602	CRF02_AG clone 266	0.51±0.06	0.16±0.02
AE	11603	CRF01_AE clone 269	0.52±0.02	0.13±0.01
B		B41	0.36±0.04	0.139±0.001
	11018	QH0692, clone 42	0.27±0.01	0.13±0.01
	11022	PVO, clone 4	0.41±0.06	0.094±0.007
	11023	TRO, clone 11	0.39±0.02	0.135±0.001
	11036	RHPA4259 clone 7	0.37±0.07	0.13±0.003
	11037	THRO4156 clone 18	0.32±0.02	0.13±0.005
	11038	CAAN5342 clone A2	0.22±0.03	0.12±0.002
	11058	SC422661.8	0.32±0.08	0.12±0.02
	11560	1006_11.C3.1601	0.58±0.06	0.135±0.002
	11561	1054.TC4.1499	0.25±0.02	0.14±0.01
	11562	1056.TA11.1826	0.58±0.01	0.13±0.01
	11563	1058 11.B11.1550b	0.29±0.02	0.17±0.01
	11572	9021_14.B2.4571	0.36±0.08	0.13±0.006
	11578	WEAUd15.410.5017b	0.76±0.04	0.13±0.004
C	11307	Du172, clone 17	0.3±0.03	0.12±0.001
	11308	Du422, clone 1	0.63±0.09	0.22±0.02
	11309	ZM197M.PB7, SVPC6	0.27±0.01	0.12±0.01
	11310	ZM214M.PL15, SVPC7	0.24±0.007	0.12±0.003
	11312	ZM249M.PL1, SVPC10	0.55±0.07	0.17±0.01
	11313	ZM53M.PB12, SVPC11	0.54±0.03	0.136±0.002
	11314	ZM109F.PB4	0.29±0.01	0.12±0.004
	11317	CAP210.2.00.E8, SVPC17	0.47±0.03	0.11±0.003
	11502	HIV-16055–2, clone 3	0.29±0.06	0.123±0.01
	11504	HIV-16936–2, clone 21	0.47±0.05	0.24±0.04
	11506	HIV-25711–2, clone 4	0.26±0.02	0.12±0.01
	11507	HIV-225925–2, clone 22	0.24±0.02	0.11±0.005
	11908	QB099.391M.ENV.B1	0.42±0.05	0.113±0.006
D	11911	QA013.70I.ENV.H1	0.32±0.01	0.12±0.01
	11912	QA013.70I.ENV.M12	0.22±0.01	0.11±0.006
	11916	QD435.100M.ENV.B5	0.3±0.01	0.11±0.01
	11918	QD435.100M.ENV.E1	0.19±0.04	0.113±0.002
G	11596	CRF02_G clone 252	0.26±0.03	0.11±0.01
Mean ± SEM (μM):	Overall (n=50) SI		0.39±0.02 108.7	0.135±0.005 293.3
	Subtype A (n=6) SI		0.42±0.05 101	0.143±0.02 277
	Subtype Arec (n=12) SI		0.44±0.03 96.4	0.139±0.009 285
	Subtype B (n=14) SI		0.39±0.04 108.7	0.131±0.004 302.3
	Subtype C (n=13) SI		0.38±0.04 111.6	0.14±0.01 282.9
	Subtype D (n=4) SI		0.26±0.03 163.1	0.11±0.002 360
Control	VSV-Gd	IC50	>20	11.9±0.5
		CC50	46.3±10.6	33.5±0.6
IC50 μM (Color Code)		≤0.2	>0.2 ≤0.5	>0.5

^aThe reported IC₅₀ values represent the means ± standard deviations (n = 3).

^bR5X4-tropic virus all the rest are CCR5-tropic viruses.

^cData previously published³²

^dVSV-G was tested in U87-CD4-CCR5 cells