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. 2021 Jan 26;21(3):236. doi: 10.3892/ol.2021.12497

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Inhibitory effect of LH on HSC-3 cell cycle. (A) Following treatment of HSC-3 cells with LH at the indicated concentrations for 12 h, the DNA content of propidium iodide-stained cells was detected using FCM; (B) total cell proportions and (C) the G₀/G₁ phase cell proportion were analyzed by FCM and GraphPad Prism 7 software, respectively. (D) Following treatment of HSC-3 cells with LH at the indicated concentrations for 24 h, the DNA content of propidium iodide-stained cells was detected using FCM; (E) total cell proportions and (F) the G₀/G₁ phase cell proportion were analyzed by FCM and GraphPad Prism 7 software, respectively. *P<0.05 was considered to indicate a statistically significant different using Dunnett's test for multiple group comparisons with the control group (0 µM LH). LH, lycorine hydrochloride.