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. 2021 Jan 26;21(3):236. doi: 10.3892/ol.2021.12497

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — LH induces apoptosis mediated by ROS in HSC-3 cells. (A) Apoptotic effects of LH on HSC-3 cells were determined via dual staining with Annexin V-FITC and PI. Alive cells are Annexin V⁻/PI⁻ (Q1-LL), cells in early apoptosis are Annexin V⁺/PI⁻ (Q1-LR), cells in late apoptosis are Annexin V⁺/PI⁺ (Q1-UR) and necrotic cells are Annexin V⁻/PI⁺ (Q1-UL). (B) Intracellular ROS levels were investigated using the ROS-detecting fluorescence dye, DCFH-DA and flow cytometric analysis. (C) Population of apoptotic cells (Q1-LR and Q1-UR) was evaluated using histogram analyses. (D) Changes in ROS were investigated via histogram analyses. *P<0.05 was considered to indicate a statistically significant difference using Dunnett's test for multiple group comparisons with the control group (0 µM LH) and Tukey's test for comparisons between group differences (groups, 20 and 20 + NAC). LH, Lycorine hydrochloride; ROS, reactive oxygen species.