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. 2021 Jan 24;21(3):225. doi: 10.3892/ol.2021.12486

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Copyright: © Yu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — MCM3AP-AS1 accelerates colorectal cancer progression via the miR-599/ARPP19 axis. (A) RT-qPCR analysis was performed to detect ARPP19 expression in SW480 cells following ARPP19-knockdown. (B) CCK-8 and (C) Transwell assays (magnification, ×200) were performed to assess the proliferation and migration of SW480 cells following ARPP19-knockdown, respectively. RT-qPCR analysis was performed to detect (D) MCM3AP-AS1, (E) ARPP19 and (F) miR-599 expression levels in SW480 cells transfected with pcDNA3.1, pcDNA3.1/MCM3AP-AS1 and pcDNA3.1/MCM3AP-AS1+miR-599. (G) CCK-8 and (H) Transwell assays (magnification, ×200) were performed to assess the proliferation and migration of SW480 cells transfected with pcDNA3.1, pcDNA3.1/MCM3AP-AS1 and pcDNA3.1/MCM3AP-AS1+miR-599, respectively. *P<0.05 vs. pcDNA3.1. miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR; CCK-8, Cell Counting Kit-8; sh, short hairpin; NC, negative control; OD, optical density.