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. 2021 Jan 20;21(3):217. doi: 10.3892/ol.2021.12478

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Copyright: © Lin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Change of the expression of tumor stem cell markers. (A) CD133⁺ MNNG/HOS cells in different groups analyzed using flow cytometry analysis. (Aa) Representative images showing the expression ratio of CD133+ cells in different groups of MNNG/HOS cells. (Ab) Changes of the expression level of CD133+ cells in different groups of MNNG/HOS cells. (B) mRNA levels of (Ba) OCT4 and (Bb) Nanog in different groups analyzed using reverse transcription-quantitative PCR analysis. Data are normalized to GAPDH and represented as relative expression (relative to control). (C) Expression of OCT4, Nanog and CD133 protein in MNNG/HOS cells cultured in different groups analyzed using western blotting. β-actin was used as an internal control. *P<0.05 and ^#P>0.05 vs. control group.