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. 2021 Jan 26;21(3):231. doi: 10.3892/ol.2021.12492

Figure 2.

Figure 2.

GATA3 suppresses HCC cell proliferation. (A) GATA3 was overexpressed or knocked down in Hep3B and MHCC97-H cells using FLAG-GATA3 plasmid or GATA3 siRNAs, respectively. After transfection for 48 h, the expression of GATA3 was determined using reverse transcription-quantitative PCR. GATA3 was (B) overexpressed or (C) knocked down in Hep3B and MHCC97-H cells using FLAG-GATA3 plasmid or GATA3 siRNAs, respectively. The expression of GATA3 was determined using western blotting. The relative densitometry of the western blots was analysed using Image J software. (D) GATA3 was overexpressed or knocked down in Hep3B and MHCC97-H cells, after which cell proliferation rate was detected using a Cell Counting Kit-8 assay. (E) After GATA3 was overexpressed or knocked down in Hep3B and MHCC97-H cells, colony formation assay was assessed to determine the effect of GATA3 on cell proliferation. *P<0.05 vs. vector; #P<0.05 siGATA3 vs. siControl. HCC, hepatocellular carcinoma; si, small interfering.