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. 2021 Feb 2;23:50. doi: 10.1186/s13075-020-02382-x

Fig. 5.

Fig. 5

a Immunohistochemical staining of collagen II at the 4th week of co-culture of TGF-β1@CS/BMP-2@SF with BMSCs (× 400). b Safranin O staining at the 4th week of co-culture of TGF-β1@CS/BMP-2@SF with BMSCs (× 200). c The GAG content increases in the presence of 15% TGF-β1@CS/BMP-2@SF detected by Alcian blue colorimetric method. d The expression of Aggrecan and collagen II in the CS/SF group normalized to β-actin determined by agarose gel electrophoresis. e The expression of Aggrecan and collagen II in the TGF-β1@CS/BMP-2@SF group normalized to β-actin determined by agarose gel electrophoresis. The results were measurement data and expressed as mean ± standard deviation derived from at least 3 independent experiments. Data comparison between two groups was analyzed by unpaired t test. n = 12