Skip to main content
. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Crit Rev Biochem Mol Biol. 2020 Dec 22;56(1):88–108. doi: 10.1080/10409238.2020.1856769

Figure 3.

Figure 3.

Structural architecture of CRISPR-Cas associated HEPN RNases. (A) Domain architecture of Cas13 subtypes A, B, C, and D. (B) Ribbon diagrams of L. buccalis Cas13a (PDB ID: 5XWP), Prevotella buccae Cas13b (PDB ID: 6DTD), and Eubacterium siraeum Cas13d (PDB ID: 6E9F) colored as seen in panel A. The HEPN RNase motifs are colored in magenta and the composite RNase active sites are boxed. The bound RNA is shown in grey. (C) Domain architecture of CRISPR-Cas associated Csm6 RNase. Abbreviation CARF defines CRISPR-associated Rossman-fold. (D) Ribbon diagram of Thermococcus onnurineus Csm6 (PDB ID: 6O6S) colored as seen in panel C.