Figure 2.

Antiviral activity comparison of C11‐6’ and P8‐6’ in vitro. a,b) These panels show on the graphs on the left the inhibitory activity of each compound against A/NL/09, superimposed with the results of the cell viability assays. Both of the compounds inhibit the virus in the dose–response assay. In the virucidal assays on the right, C11‐6’ reduced the virus titer by 1000 times, whereas the infection was fully recovered in the case of P8‐6’. Hence C11‐6’ has an irreversible inhibitory effect on the virus while the effect of P8‐6’ is reversible. c) Virucidal activity of C11‐6’ against other influenza strains was further investigated confirming its irreversible activity independently of the strain.

Note that in the figure's axes ffu stands for focus forming units and NT for non‐treated. In Figure 2c the following viral strains were tested: A/Singapore/37/2004 (H3N2), B/Wisconsin/01/2010 and A/Switzerland/8337/2018‐MDCK1 (H1N1) Figure 2c. Results are mean and SEM of 2 independent experiments performed in duplicate.