Figure 4.

Effect of virus maturation on the CT_751–811 dependent gp41 interaction with chol in purified viral particles. a) Schematic illustration of mature and immature viral particles containing either the full‐length wild‐type gp41 or the truncated ΔCT₇₅₀ gp41. b) Gp41 from immunoprecipitation from mature or immature viral particles were detected by Western blot with the anti‐gp41 chessie‐8 antibody (green lane), and the interacting radioactively labeled lipid by autoradiography of the same membrane (gray lane). c) Quantification of the chol/protein signal ratio of the wild‐type and truncated ΔCT₇₅₀ gp41 proteins in mature and immature viral particles. The bars and whiskers represent the mean ± SD of experiments from three independent viral purifications (n = 3). Statistical significance was assessed by analysis of variance and Tukey test.