Fig. 5. Neutralization of live virus by designed miniprotein inhibitors.

(A and B) Neutralization activity of (A) AHB1 and AHB2 or (B) LCB1-5 were measured with a focus reduction neutralization test. Indicated concentrations of minibinders were incubated with 100 FFU of authentic SARS-CoV-2 and subsequently transferred onto Vero E6 monolayers. AHB1, AHB2, LCB1, and LCB3 potently neutralize SARS-CoV-2, with median effective concentration (EC₅₀) values <50 nM (AHB1 and AHB2) or <50 pM (LCB1 and LCB3). Data are representative of two independent experiments, each performed in technical duplicate.