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. 2020 Dec 20;22(2):e51239. doi: 10.15252/embr.202051239

Figure EV2. EV characterization by density gradient fractionation.

Figure EV2

  • A, B
    Western blot and density measurement of EV fractions collected from indicated cell lines to detect EV markers.
  • C, D
    RT–qPCR of EV fractions collected from indicated cell lines to detect miR‐105 and miR‐204 levels (n = 3 biological replicates for each fraction). Data are presented as mean ± SD. ND: not detected.
  • E
    A schematic representation of the density gradient separation procedure to further characterize the 110,000 × g pellets.

Source data are available online for this figure.