Figure 3. Effects of cancer‐derived EVs on AA consumption by fibroblasts.
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A, BDecreases of leucine, arginine, and lysine (A) as well as production of ammonium (B) in the conditioned medium (CM) of NIH3T3 cells at 30 min after the LRK mixture was added to starved cells that were pretreated with EVs or PBS (n = 3 biological replicates for each group).
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CIntracellular levels of leucine, arginine, and lysine in NIH3T3 cells collected 30 min after the LRK mixture was added to starved cells (n = 3 biological replicates for each group).
Data information: In (A–C), data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 (one‐way ANOVA). † not detected.