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. 2021 Jan 7;22(2):e51127. doi: 10.15252/embr.202051127

Table 1.

Information for CRISPR/Cas9 and genotyping of 53bp1 andUsp28 mouse alleles

53bp1 Usp28
Exon 4 2
gRNA TCTTCTCATTTGGGTACCAG AATCAGCTGCGAGAAATCAC
Mutation 5 bp deletion and 4 bp insertion (a net of 1 bp deletion) 1 bp insertion
InDel TCTTCTCATTTGTTCT‐CAG AATCAGCTGCGAGAAATTCAC
Primer 1 GTGTTTAAGGTCCTGTGGGG TGATGCTCTGCTCCGAGAAA
Primer 2 AGCTTTAATGTCCCTGCCCA AAGCCCACTGTACATTCCCA