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Information for CRISPR/Cas9‐generated p53 alleles in mESCs
| p53 | ||||
|---|---|---|---|---|
| Exon | 4 | |||
| gRNA | AGGAGCTCCTGACACTCGGA | |||
| Cell line | p53 −/− | Sas‐4 −/− p53 −/− | ||
| Mutation | 16 bp deletion | 1 bp deletion | 2 bp deletion | 1 bp deletion |
| InDel |
TCC‐‐‐‐‐‐‐ ‐‐‐‐‐‐‐‐‐T |
T‐CGAGTGTC AGGAGCTCCT |
T‐‐GAGTGTC AGGAGCTCCT |
TCC‐AGTGTC AGGAGCTCCT |
| Primer 1 | TTGTTTTCCAGACTTCCTCCA | |||
| Primer 2 | CTGAAGAGGAACCCCCAAAT | |||
