Total cell lysates from wild‐type (WT) and HAT1 knockout (KO) Clone 1 HepG2 cells were analyzed for the levels of Ksucc and Kac by Western blot analysis (left). The expression levels of HAT1, Histone H3 (H3), and beta‐actin (β‐actin) were measured by Western blot analysis in the cells (right).
Quantitative proteomics of lysine (K) succinylation was performed in HepG2 and HAT1 knockout HepG2 cells. Graphs show the overlap of the total number of succinylated sites (left) and proteins (right) identified with those targeted by HAT1 (> 1.2‐fold decrease and P < 0.05, Student's t‐test).
Volcano plot depicting the relative fold change of Ksucc levels in the succinylation quantitative proteomics of WT versus HAT1 KO cells. The vertical blue, dashed lines represent the threshold of 1.2‐fold, and the horizontal blue, dashed lines represents the threshold of P = 0.05. Student's t‐test.
The diagram showing the cellular distribution of succinylated proteins targeted by HAT1 identified in the quantitative succinyl‐proteome.
GSEA analysis showing the GO processes for the succinylated proteins targeted by HAT1 in the succinylation quantitative proteomic. BP, biological process; CC, cellular component; MF, molecular function.
