Figure 7. Sugarbabe is a downstream effector of ERK7 and PWP1.

• A, B
  Expression of sugarbabe is downregulated in fat bodies upon knockdown of PWP1 (A) and ectopic expression of ERK7 (B) (N = 3 replicates of 10 fat bodies/replicate for each genotype).
• C
  ERK7 ¹ mutants fail to maximally downregulate sugarbabe expression upon fasting (N = 4 replicates of 10 larvae/replicate for each genotype).
• D
  Representative immunofluorescent images of fat bodies in a genetic epistasis experiment using ERK7 ¹ and sug mutants (sug ^17Δ/Df(2R)Exel7123) with LipidTOX and DAPI staining to visualize lipid droplets and nucleus, respectively. Scale bar: 50 µm.
• E
  sug mutant (sug ^17Δ/Df(2R)Exel7123) suppresses increased lipid droplet number observed in ERK7 ¹ mutants (N > 20).
• F
  Ectopic sugarbabe expression in the fat body partially rescues TAG levels in the CG>ERK7 larvae (N = 4 replicates of ≥10 larvae/replicate for each genotype).
• G
  sug mutant (sug ^17Δ/Df(2R)Exel7123) suppresses increased nuclear size phenotype of ERK7 ¹ mutant fat body cells (N ≥ 25 cells per genotype).
• H, I
  Ectopic Sugarbabe expression in the fat body partially restores the pupariation rate (H) (P < 0.001 for the rescue, analyzed by Log‐rank test, N = 4 replicates of 30 larvae/replicate for each genotype) and pupal volume of CG>ERK7 larvae (I) (N = 4 replicates of 10 pupae/replicate for each genotype).
• J
  Addition of 20% sucrose improves the growth rate and survival of CG>ERK7 larvae. Pupariation kinetics of CG>ERK7 larvae on 20% yeast or 20% yeast + 20% sucrose diet (P < 0.0001 for the rescue, analyzed by Log‐rank test, N = 4 replicates of 30 larvae/replicate for each genotype and diet).

Data information: N stands for the number of biological replicates. Error bars display standard deviation (SD). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t‐test). Expression of RP49 was used for normalization of (A–C).