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. 2021 Jan 5;22(2):e50927. doi: 10.15252/embr.202050927

Figure EV4. Thymidine‐induced cell cycle arrest in primary mouse epithelial cells and HEC151 endometrial epithelial cell line.

Figure EV4

  • A
    Thymidine‐induced cell cycle arrest in primary mouse epithelial cells. Mouse uterine epithelial cells were stained with propidium iodine, and cell fractions were quantified by flow cytometry.
  • B, C
    Annexin V assay using a human endometrial epithelial cell line HEC151 showed that TNFα administration increases the expression of annexin V in growth‐arrested HEC151 cells with thymidine treatment, but does not in the control cells (mean ± SEM, Student’s t‐test). The assay was performed in duplicate for each group, and the examination was performed three times.
  • D
    The expression of TNF receptor type 2 (TNFR2) was upregulated in growth‐arrested HEC151 cells with thymidine treatment (mean ± SEM, Student’s t‐test). Quantitative PCR was performed in quadruplicate for each group.