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. 2021 Jan 5;22(2):e51524. doi: 10.15252/embr.202051524

Figure 6. Abnormal cortical structures in offspring brains derived from aged fathers in the early postnatal period.

Figure 6

  1. Representative images of the cerebral cortex of YFO (left) and AFO (right) at postnatal day 6. Scale bar: 1 mm.
  2. Cortical thickness in the postnatal brain of YFO (n = 4, from different mothers) and AFO (n = 5, from different mothers).
  3. Immunostaining for cortical layer markers in the postnatal brain of YFO and AFO (representative magnified images of the white boxes in panel A). Satb2+/Ctip2−: layer 2–4, Ctip2high: layer 5, Satb2−/Ctip2−: layer 6. Scale bar: 100 μm.
  4. Immunostaining for another cortical layer markers in the postnatal brain of YFO and AFO (representative magnified images of the white boxes in panel A). Tbr1−/Ctip2−: layer 2–4, Ctip2high: layer 5, Tbr1+/Ctip2−: layer 6. Scale bar: 100 μm.
  5. Layer thickness (mm) in each cortical layer in the postnatal brain of YFO (n = 4, from different mothers) and AFO (n = 5, from different mothers).
  6. Cell number in each cortical layer in the postnatal brain of YFO and AFO.
  7. Cell density in each cortical layer in the postnatal brain of YFO and AFO.

Data information: All data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, determined using Student t‐test.